Antioxidant supplementation ameliorates bull sperm parameters and fertilizing ability following the freeze-thaw process

This study's goal was to reveal the effects of antioxidant supplementation on motility, motion characters, morphology, DNA integrity, and fertilizing potential cryopreserved bovine sperm. The ejaculates were collected from three Holstein bulls. At least ten per bull. immediately separated into five aliquots, diluted in Cryobos (Magapor Co. Ltd., Zaragoza, Spain) commercial extender, including 2.5 mM taurine, cysteine, methionine, glutamine, no additives used. Afterward, they frozen 0.25 mL French straws, liquid nitrogen used for storage semen. methionine resulted a higher subjective motility rate comparison with other groups. Taurine led lowest post-thawed CASA (p < 0.05). addition antioxidants did not cause any improvements sperm characteristics when compared controls > Cysteine protection acrosome abnormality, groups For comet test, minimum percentage damaged obtained cysteine glutamine There significant difference among terms pregnancy rates In conclusion, semen extender prior freezing ameliorated quality.